SD/-Ade/-His/-Leu/-Trp/-Met Broth - Names and Identifiers
SD/-Ade/-His/-Leu/-Trp/-Met Broth - Physico-chemical Properties
Storage Condition | Room Temprature |
SD/-Ade/-His/-Leu/-Trp/-Met Broth - Introduction
SD/-Ade/-His/-Leu/-Trp/-Met Broth (also known as SD/-Ade/-His/-Leu/-Trp/-Met soup) is a commonly used medium for the cultivation and screening of bacteria or yeast.
Properties of SD/-Ade/-His/-Leu/-Trp/-Met Broth:
-It is a non-nutrient medium, that is, it does not contain nutrients such as glucose or nitrogen sources.
-The lack of adenine (Ade), histidine (His), leucine (Leu), tryptophan (Trp) and methionine (Met) in the medium requires the addition of additional glucose and nitrogen sources to meet growth requirements.
SD/-Ade/-His/-Leu/-Trp/-Met Broth Usage:
-Used for selective cultivation and screening of expression strains of affinity tag (such as His, Leu, Trp, etc.) proteins. When strains have the required affinity tags, they are able to grow in this medium, whereas other strains that do not have these tags are unable to grow.
-It is used to construct genome engineering experiments such as anabolic pathways, with high flexibility.
SD/-Ade/-His/-Leu/-Trp/-Met Broth:
-Dissolve an appropriate amount of non-nutrient medium powder in distilled water and prepare according to the concentration given in the instructions.
-Add additional nutrients, such as glucose and nitrogen sources, according to experimental requirements.
-Adjust the pH to the desired range, usually pH 5.8-6.0.
-Sterilization can be done by high temperature and high pressure sterilization or filtration sterilization.
Safety information for SD/-Ade/-His/-Leu/-Trp/-Met Broth:
-the use of culture medium to comply with the laboratory safety procedures, such as the correct use of laboratory supplies, wear personal protective equipment, etc.
-Proper disposal of expired or discarded media in accordance with fire and waste regulations.
-Adjust the composition of the medium according to the experimental conditions to ensure safety and effectiveness.
-In the preparation and use of the process, pay attention to avoid contact with the culture medium and skin or mucous membrane, such as accidental contact should immediately rinse with plenty of water. If you feel unwell, seek medical help.
Last Update:2024-04-09 19:05:59